Applications: | Enzyme Activity, SDS-PAGE |
Species Reactivity: | E.coli |
MW: | 21 kDa (196 aa), confirmed by MALDI-TOF. |
Purity: | > 95% by SDS - PAGE |
Description: | SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E. Coli slyD was expressed in E. coli and purified by conventional chromatography. |
Further Information: | Sequence: MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH |
Synonym: | FKBP-type peptidyl-prolyl cis-trans isomerase, FKBP-type peptidyl-prolyl cis-trans isomerase, SlyD, FKBP-type peptidyl-prolyl cis-trans isomerase FKBP type peptidyl prolyl cis trans isomerase slyD, Histidine rich protein, PPIase, Rotamase, WHP. |
Ascension Number: | P0A9K9 |
NCBI EntrezGene: | NP_755987 |
Shipping Conditions: | Ship on cold packs |
Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Usage: | Research use only |