Ordering Details
Cat Number:	RT001-0050
Size:	50 rxns
Concentration:	10,000 units/50uL
Price:	£111.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

RScript Reverse Transcriptase 10,000U

Partner: Bio-Helix Co., Ltd

Description:	Bio-Helix RScript Reverse Transcriptase - Engineered innovatively and specifically for both Research and Diagnostic applications for meeting all your cDNA synthesis needs and for overcoming the most challenging secondary RNA structures over a wide temperature range. Our next-generation, engineered recombinant M-MLV reverse transcriptase, with improved thermostability, processivity, robustness, optimal cDNA yields, proprietary site mutations for reduced RNase H activity, and extended half-life, is the most versatile reverse transcriptase in the world for not only simply meeting the routine cDNA synthesis requirements but also enabling superior performance for even the most challenging RNA samples at hand.
Shipping Conditions:	Ship on freezer packs
Storage:	Store at -20 C
Usage:	Research use only

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Elongation of RNA templates up to 11 kb in length: RScript reverse transcriptase was used in a reaction with a range of human bladder cancer cell lines - 5637 (HTB-9) RNA. The resulting synthesized cDNA (8 kb ,11kb) was followed by PCR and visualized on a 2% agarose gel. The molecular weight marker used was DNA Ladder 1kb #SDL-1000R.

Performance of One-Step RT-qPCR with RScript reverse transcriptase: To evaluate the RT performance for the COVID-19 fragments, the N1 gene with 100 copies per reaction was tested in the one-step RT-qPCR. Experiments proved to be feasible and effective (mean Ct : 34.88).