Elongation of RNA templates up to 11 kb in length: RScript reverse transcriptase was used in a reaction with a range of human bladder cancer cell lines - 5637 (HTB-9) RNA. The resulting synthesized cDNA (8 kb ,11kb) was followed by PCR and visualized on a 2% agarose gel. The molecular weight marker used was DNA Ladder 1kb #SDL-1000R.
Performance of One-Step RT-qPCR with RScript reverse transcriptase: To evaluate the RT performance for the COVID-19 fragments, the N1 gene with 100 copies per reaction was tested in the one-step RT-qPCR. Experiments proved to be feasible and effective (mean Ct : 34.88).