HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GNE (Cat# RC222626, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNE antibody(Cat# TA890043). Positive lysates [LY417278] (100ug) and [LC417278] (20ug) can be purchased separately from OriGene.