HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HLCS (Cat# RC209197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLCS(Cat# TA504422). Positive lysates [LY400145] (100ug) and [LC400145] (20ug) can be purchased separately from OriGene.