HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TNFRSF14 (Cat# RC201167, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF14 (Cat# TA808093)(1:2000). Positive lysates [LY401254] (100ug) and [LC401254] (20ug) can be purchased separately from OriGene.