Western blot of rabbit anti-Ferritin antibody. Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1/1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa/20 kDa for Ferritin. Other band (s): None.