Shown here is a serial 1/1 dilution of Control Rabbit IgG protein (Cat.-No RA032, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG Cat.-No R1454HRP and Dylight 649 conjugated Goat anti Rabbit IgG 1/20K in MB-070. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager.
