Western Blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody: Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH/3T3 Whole Cell Lysate. Load: 10ug per lane. Primary antibody: Beta Actin Antibody at 1/1000 for overnight at 4°C. Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT .Predicted/Observed size: 42 kDa.