Goat anti Sucrose phosphorylase antibody was used to detect purified Sucrose phosphorylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1/3000 dilution of primary antibody (on 4°C in blocking buffer). Detection shown was using Dylight 488 conjugated donkey anti goat secondary antibody. Images were collected using the BioRad VersaDoc System.
