Western blot of rabbit anti-Ovalbumin peroxidase conjugated antibody. Lane 1: Ovalbumin (hen egg). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ovalbumin peroxidase conjugated antibody at 1/1,000 overnight at 4°C. Secondary antibody: none. Blocking buffer for 30 min at RT. Predicted/observed size: 43 kDa for Ovalbumin. Other band (s): Ovalbumin splice variants and isoforms.