<b>Immunofluorescence Microscopy</b> of Anti-GFP (GOAT) Antibody: Tissue: E5.5 Hex-GFP transgenic mouse embryo. Primary antibody: Goat anti-GFP was used at 1/500 dilution. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody at 1/10,000 for 45 min at RT. Staining: GFP as green fluorescent signal with DAPI blue counterstain.