Western Blot of Rabbit anti-Glucose Oxidase Antibody Peroxidase Conjugated. Lane 1: Glucose Oxidase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Glucose Oxidase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 66 kDa, 66 kDa for Glucose Oxidase. Detected using FEMTOMAX-110 substrate.