Goat anti Glucose Dehydrogenase antibody. was used to detect purified Glucose Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1/3,000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated secondary antibody. Images were collected using the BioRad VersaDoc System.