Western blot of peroxidase conjugated rabbit anti-Carboxypeptidase B antibody. Lane 1: Carboxypeptidase B. Load: 50 ng per lane. Primary antibody: Peroxidase conjugated rabbit anti-Carboxypeptidase B antibody at 1/1,000 overnight at 4°C. Secondary antibody: n/a. Blocking buffer for 30 minutes at RT. Predicted/Observed size: 47 kDa, observed at 35 kDa for Carboxypeptidase B. Expected molecular weight for processed protein is ~34.7 kDa.