Western blot of rabbit anti-Beta-galactosidase (E. coli) antibody biotin conjugated. Lane 1: Beta-galactosidase. Load: 50 ng per lane. Primary antibody: Beta-galactosidase (E. coli) antibody biotin conjugated. R1064B) at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer ( for fluorescent Western blotting) for 30 min at RT. Predicted/observed size: 116 kDa, 116 kDa for Beta-galactosidase.