Western blot of rabbit anti-L-asparaginase antibody. Lane 1: L-asparaginase. Lane 2: none. Load: 100 ng per lane. Primary antibody: L-asparaginase antibody at 1/1,000 for overnight at 4°C. Secondary antibody: DyLight?TM 649 rabbit secondary antibody at 1/20,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/observed size: 32 kDa for L-asparaginase. Other band (s): L-asparaginase splice variants and isoforms.