Western blot of peroxidase conjugated rabbit anti-L-asparaginase antibody. Lane 1: L-asparaginase. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking buffer for 30 min RT. Predicted/observed size: 30 kDa for L-asparaginase. Other band (s): L-asparaginase splice variants and isoforms.