Western blot of biotin conjugated rabbit anti L-asparaginase antibody. Lane 1: L-asparaginase. Lane 2: none. Load: 100 ng per lane. Primary antibody: Biotin conjugated L-asparaginase antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/observed size: 32 kDa for L-asparaginase. Other band (s): L-asparaginase splice variants and isoforms.