Western Blot of Rabbit anti-L-Asparaginase Antibody. <br /><strong>Lane 1:</strong> L-Asparaginase. <strong>Lane 2:</strong> none. <strong>Load:</strong> 100 ng per lane. <strong>Primary antibody:</strong> L-Asparaginase antibody at 1/1000 for overnight at 4°C. Secondary antibody: DyLight™ 649 Rabbit secondary antibody at 1/20,000 for 30 min at RT. <strong>Block:</strong> MB-070 for 30 min at RT. <strong>Predicted/Observed size:</strong> 32 kDa for L-Asparaginase. Other band(s): L-Asparaginase splice variants and isoforms.