To detect mIP-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with the Biotinylated Anti-Murine IP-10 PP1083B1/PP1083P2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIP-10.