| Applications: | Dot Blot, ELISA, WB |
| Species Reactivity: | Human |
| Isotype: | Rabbit IgG |
| Immunogen: | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion surrounding T244 of Human CDC27. |
| Purification: | This affinity purified antibody is directed against the phosphorylated form of human CDC27 at the pT244 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human CDC27 pT244 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human CDC27 is minimal by ELISA. The antibody does not cross-react with CDC27 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, bovine and chimpanzee based on 100% homology for the immunogen sequence. Cross-reactivity with CDC27 protein from mouse, rat and chicken may occur as sequence homology varies by one amino acid residue in this sequence (90% homology). Cross-reactivity with CDC27 homologues from other sources has not been determined. |
| Description: | Boster Bio Anti-CDC27 phospho T244 Antibody (Catalog # P03905). Tested in Dot Blot, ELISA, WB applications. This antibody reacts with Human. |
| Synonym: | CDC27 pT244, CDC 27, CDC-27, Cell division cycle protein 27 homolog, H-NUC, APC3, Anaphase-promoting complex subunit 3, ANAPC3 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Store vial at -20 C prior to opening. Aliquot contents and freeze at -20 C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. |
| Usage: | Research use only |
