Applications: | ELISA, IHC, WB |
Species Reactivity: | Human, Monkey |
Isotype: | Rabbit IgG |
Immunogen: | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein. |
Purification: | Anti-Phospho Aurora B pT232 affinity purified antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross-reactivity with Aurora Kinase B from other sources is not known. |
MW: | 39384 MW |
Description: | Boster Bio Anti-Phospho-Aurora B pT232 Aurkb Antibody (Catalog # P00762). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey. |
Synonym: | Aurora B pT232, Phospho Aurora B, AIK2, AIM1, ARK2, AurB, AURKB, Aurora 1, Aurora and Ipl1 like midbody associated protein 1 |
Shipping Conditions: | Ship on cold packs |
Storage: | Store Phospho Specific Antibody at -20 C prior to opening. Aliquot contents and freeze at -20 C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. |
Usage: | Research use only |