Description: | The EpiQuik Total RNA Isolation Fast Kit provides a fast, simple, and cost-effective method for the isolation of total RNA from mammalian cells and tissues. Detergents are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows protein/DNA to be removed and RNA to bind to the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted. The RNA purified with this kit is suitable for a variety of routine applications, including RT-PCR, cDNA synthesis, northern blotting, differential display, primer extension, and mRNA selection. The entire procedure can be completed within 25-40 minutes. The kit has the following features and advantages:Fast procedure delivers high-quality total RNA in approximately 30 minutesReady-to-use RNA for high performance in any downstream applicationConsistent RNA yield from a small amount of starting material |