| Description: | The Epigenase m6A Methylase Activity/Inhibition Assay Kit (Colorimetric) is a complete set of optimized buffers and reagents designed for measuring the activity/inhibition of total m6A methylases (methyltransferases) using nuclear extracts or purified m6A methylases like METTL3/METTL14 from a broad range of species such as mammalian, plant, fungal, and bacterial, in a variety of forms including, but not limited to, cultured cells and, fresh and frozen tissues. This kit has the following advantages and features:Fast: Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours.Robust: Innovative kit composition enables background signals to be extremely low and allows the assay to be simple, accurate, reliable, and consistent.Convenient: Both cell/tissue nuclear extracts and purified proteins can be used, which allows detection of inhibitory effects of m6A methylase inhibitor in vivo and in vitro.Sensitive and Specific: Novel assay principle allows to detect the m6A methylase-converted end product, which makes the assay much more specific than that for by-product measurement. The assay also allows high sensitivity to be achieved. The activity can be detected from as low as 2 ug of nuclear extracts or 50 ng of purified enzymes.Quantitative: The assay standard is included, which allows the specific activity of m6A methylase to be quantified. Flexible: Strip-well microplate format makes the assay flexible for manual or high throughput analysis. |
