| Description: | The EpiQuik CUT&RUN m6A RNA Enrichment Kit is a complete set of optimized buffers and reagents designed to enrich an RNA fragment containing m6A from low input RNA and to identify region-specific m6A by PCR or profile epitranscriptome-wide m6A by next-generation sequencing using Illumina platforms or other methods. The innovative working principle, optimized protocol, and components of the kit allow for the capture of the m6A fragment with minimal non-specific background levels. The enriched RNA is specifically suitable to construct both non-barcoded (singleplexed) and barcoded (multiplexed) libraries quickly, allowing m6A regions to be mapped with less bias and at a high resolution. The kit has the following features: High enrichment: Uses an RNA cleavage enzyme mix to simultaneously fragment RNA and cleave/remove any RNA sequences in both ends of the target m6A-containing sequences without affecting RNA regions occupied by the antibody. Short RNA fragments are generated only bound with anti- m6A antibody. True target m6A-enriched regions can, therefore, be reliably identified, and high-resolution mapping achieved.Low input: Unbound RNA cleavage and immunocapture are processed in the same single-tube, which enables maximum protection of the target m6A-containing regions and minimized sample loss, allowing the input RNA to be as low as 500 ng.Minimal Background: Cleavage of unbound RNA sequences in the two (2) ends of the target m6A-containing sequences enables minimized MeRIP/sequencing background, allowing data analysis with <10 million reads.Fast, streamlined procedure: The procedure from RNA to library cDNA is less than 3 hours with <30 min of hands-on timeHighly convenient: The kit contains all required components for each step of the EpiQuik CUT&RUN m6A RNA Enrichment Kit, which are sufficient for m6A-containing RNA sequence capture, thereby allowing CUTARUNTM m6A RNA enrichment to be the most convenient with reliable and consistent results. |
