| Description: | The EpiQuik In Vivo HDAC1 Sumoylation Assay Kit is designed for measuring sumoylation of targeted proteins. Sumoylation of HDAC1 is indicated by SUMO conjugated to this protein. In assay with this kit, the antibody specific for the HDAC1 is stably coated on the strip wells. HDAC1 contained in the nuclear extracts is captured by the antibody. Sumoylation of HDAC1 can then be detected by recognition of SUMO conjugated to HDAC1 with an anti-SUMO antibody. The ratio or intensity of the sumoylation, which is proportional to the conjugated SUMO amount, can be quantified through the signal report-color development system. |
