Schematic procedure for using the EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit.
Histone extracts were prepared from MDA-231 cells using the EpiQuik Total Histone Extraction Kit (Cat. No. OP-0006) and the amount of dimethyl-H3-K4 was measured using the EpiQuik Dimethyl Histone H3-K4 Quantification kit (Colorimetric).
HSC-3 cells were incubated with/without zebularine (220 mM) for 48 hours. Nuclear proteins were extracted and total DNMT activity was measured.