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Cat Number:P-2030-12
Size:each
Price:£519.00
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Shipping:£10.00

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EpiNext ChIP-Seq High-Sensitivity Kit [Illumina] [12 reactions]

Partner: Epigentek Group Inc

Applications:IP, Library Construction
Description:The EpiNext ChIP-Seq High-Sensitivity Kit (Illumina) is a complete set of reagents required for carrying out a successful ChIP-Seq starting from mammalian cells or tissues. The kit is designed to selectively enrich a chromatin fraction containing specific DNA sequences from various species, particularly mammals, and to prepare a ChIP-Seq library for next generation sequencing using Illumina platforms such as Illumina Genome Analyzer II, HiSeq and MiSeq systems. The optimized protocol and components of the kit allow capture of low abundance protein/DNA complexes with minimized non-specific background levels and the ability to construct both non-barcoded (singleplexed) and barcoded (multiplexed) ChIP-Seq libraries quickly with reduced bias.
Shipping Conditions:Ship on cold packs
Usage:Research Use Only

 

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Workflow of the   EpiNext� ChIP-Seq High-Sensitivity Kit (Illumina).

Workflow of the EpiNext� ChIP-Seq High-Sensitivity Kit (Illumina).

Highly sensitive ChIP: The sheared chromatin isolated from different amounts of MBD-231 cells was  used for ChIP-qPCR analysis of RNA polymerase II enrichment in GAPDH promoters.

Highly sensitive ChIP: The sheared chromatin isolated from different amounts of MBD-231 cells was used for ChIP-qPCR analysis of RNA polymerase II enrichment in GAPDH promoters.

Size distribution of library fragments. Ten nanograms of DNA was ChIPed by RNA polymerase II enrichment and used for DNA library preparation.

Size distribution of library fragments. Ten nanograms of DNA was ChIPed by RNA polymerase II enrichment and used for DNA library preparation.