Ordering Details
Cat Number:	P-1015-24
Size:	each
Price:	£375.00
Quantity:
Shipping:	£12.00

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Data Sheet

Methylamp Methylated DNA Capture [MeDIP] Kit [24 reactions]

Partner: Epigentek Group Inc

Applications:	IP
Description:	The Methylamp Methylated DNA Capture (MeDIP) Kit is a complete set of optimized reagents to enrich and capture methylated DNA fragments in a convenient microplate-based format. The method, methylated DNA immunoprecipitation (meDIP), uses a monoclonal antibody specific to 5-methylcytosine to immunoprecipitate methylated genomic DNA. The enriched methylated fractions can then be used for gene-specific DNA methylation analysis on a genome wide scale. The Methylamp Methylated DNA Capture Kit is suitable for combining the specificity of enriched methylated DNA with qualitative and quantitative PCR, traditional sequencing and next-generation sequencing, DNA microarrays, as well as Southern blot
Further Information:	Please see the EpiQuik Methylated DNA Immunoprecipitation Kit if you are working with nuclear extract as the starting material.
Shipping Conditions:	Ship on cold packs
Usage:	Research Use Only

Insight Biotechnology Ltd reserves the right to change pricing without notice

Schematic procedure of the Methylamp Methylated DNA Capture Kit.

For enrichment of methylated DNA using the kit, DNA (0.5 ug) isolated from MCF-7 cells was added into the microwell. Methylated DNA was captured by 5-mC antibody prebound to the microwells.

Captured methylated DNA was used for analyzing methylation level of GAPDH and MLH1 promoter with the use of primers and probes specific to GAPDH and MLH1 promoters, respectively.