| Applications: | WB, ICC, IF, IP |
| Species Reactivity: | Human, Mouse, Rat, Sheep, Rabbit, Hamster, Dog, Bovine |
| Dilutions: | WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
| Isotype: | Rat |
| Immunogen: | Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli. Detects the COOH group. |
| Purification: | Protein G Purified |
| Conjugation: | Unconjugated |
| Description: | T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL. |
| Shipping Conditions: | Ship on cold packs |
| Usage: | Research Use Only |
