| Applications: | FC, IHC, IP, WB, BL, CMCD |
| Dilutions: | Flow Cytometry: Fluorescein conjugate<br/>Biotin conjugate<br/>PE, APC, SPRD, CY5, and AF647<br/>conjugates<br/><= 3 ug/10<sup>6</sup> cells<br/><= 1 ug/10<sup>6</sup> cells<br/><= 0.1 ug/10<sup>6</sup> cells |
| Isotype: | Hamster IgG |
| Immunogen: | H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37 |
| Conjugation: | Unconjugated |
| Shipping Conditions: | Ship on dry ice |
| Storage: | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C<br/>- The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg or 0.1 mg in 1.0 mLof PBS/NaN3. Store at 2- 8 C<br/>- The biotin (BIMA) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C<br/>- The R- phycoerythrin (R- PE), allophycocyanin (APC), and Spectral Red (SPRD) conjugates aresupplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C<br/>Do not freeze!<br/> - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C<br/>- The Alexa Fluor 647 (AF647) conjugate is supplies as 0.1 mg in 0.2mL of PBS/NaN3. Store at 2- 8 C<br/>- Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Usage: | Research Use Only |
