<strong>Left:</strong> Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Aurora A. Nocodazole is a microtubule poymerization inhibitor which induces cells to halt at G2/M phase and induces Aurora A expression. The Aurora A monoclonal binds strongly and cleanly to a band at about 46 kDa. <br /><strong>Right:</strong> Blot of recombinant human Aurora A, B and C was probed with Aurora A. MO22137 recognized only Aurora A protein.