| Description: | <h2>Product Overview</h2><p>The Multiplex Porcine Cytokine � High Sensitivity (4-plex) is a fully quantitative, ELISA-based chemiluminescent assay allowing concurrent measurement of cytokines in serum or plasma samples.</p><p>A kit contains a 96-well plate, with each well featuring the 4-plex along with a positive control with all the other reagents necessary to perform the test. Our high-quality reagents help ensure the accuracy of your results.</p><p>Using just 50 �l of sample per well, up to 80 samples can be assayed for all four markers in the panel in 4.83 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.</p> <h2>Assay Ranges</h2><table class=attributes> <thead><tr><th></th><th></th><th></th><th></th></tr></thead><tbody><tr ><td></td><td> Units </td><td> Calibrator Range </td><td> LLD Limit </td></tr><tr ><td> IL-1? </td><td> pg/ml </td><td> 1550 - 2.13 </td><td> 2.10 </td></tr><tr ><td> IL-6 </td><td> pg/ml </td><td> 1800 - 2.47 </td><td> 2.50 </td></tr><tr ><td> IL-8 </td><td> pg/ml </td><td> 2100 - 2.88 </td><td> 2.90 </td></tr><tr ><td> TNF? </td><td> pg/ml </td><td> 1600 - 2.19 </td><td> 1.90 </td></tr></tbody></table> <h2>Kit Components</h2><p>Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.</p><p>Reagents Include:</p><p>Calibrator: Lyophilized, recombinant antigens in a buffered protein base<br>Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives <br>Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide <br>Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer <br>Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives <br>Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase<br>Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant<br>Plate Seals (20): Adhesive strips</p> |
