| Description: | <h2>Product Overview</h2><p>The Multiplex Mouse Cytokine Panel 2 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IFN?, IL-1?, IL-6, TNF?).</p><p>Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.</p><p>Using just 50 �l of sample per well, up to 80 samples can be assayed for all four markers in the panel in 2.25 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.</p> <h2>Assay Ranges</h2><table class=attributes> <thead><tr><th></th><th></th><th></th><th></th></tr></thead><tbody><tr ><td></td><td> Units </td><td> Calibrator Range </td><td> LLD Limit </td></tr><tr ><td> IFN-? </td><td> pg/ml </td><td> 8000 - 10.97 </td><td> 5.40 </td></tr><tr ><td> IL-1? </td><td> pg/ml </td><td> 14000 - 19.20 </td><td> 12.41 </td></tr><tr ><td> IL-6 </td><td> pg/ml </td><td> 5000 - 6.86 </td><td> 2.90 </td></tr><tr ><td> TNF? </td><td> pg/ml </td><td> 3000 - 4.12 </td><td> 3.40 </td></tr></tbody></table> <h2>Kit Components</h2><p>Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.</p><p>Reagents Include:</p><p>Calibrator: Lyophilized, recombinant antigens in a buffered protein base<br>Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives <br>Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide <br>Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer <br>Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives <br>Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase<br>Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant<br>Plate Seals (20): Adhesive strips</p> |
