| Description: | <h2>Product Overview</h2><p>The Multiplex Human Cytokine HS (9-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 9 biomarkers or analytes. Using less than 50 �l of sample per well, up to 80 samples can be assayed for all 9 markers in the panel within 2.25 hours. The Multiplex Human Cytokine HS (9-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 9 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.</p> <h2>Assay Ranges</h2><table class=attributes> <thead><tr><th></th><th></th><th></th><th></th></tr></thead><tbody><tr ><td></td><td> Units </td><td> Calibrator Range </td><td> LLD Limit </td></tr><tr ><td> IL-1? </td><td> pg/ml </td><td> 4000 � 5.49 </td><td> 5.43 </td></tr><tr ><td> IL-1? </td><td> pg/ml </td><td> 10000 � 13.72 </td><td> 10.14 </td></tr><tr ><td> IL-2 </td><td> pg/ml </td><td> 4000 � 5.49 </td><td> 3.16 </td></tr><tr ><td> IL-4 </td><td> pg/ml </td><td> 2000 � 2.74 </td><td> 2.14 </td></tr><tr ><td> IL-6 </td><td> pg/ml </td><td> 3000 � 4.11 </td><td> 3.47 </td></tr><tr ><td> IL-8 </td><td> pg/ml </td><td> 2000 � 2.74 </td><td> 2.70 </td></tr><tr ><td> IL-10 </td><td> pg/ml </td><td> 3000 � 4.11 </td><td> 4.10 </td></tr><tr ><td> IFN? </td><td> pg/ml </td><td> 8000 � 10.97 </td><td> 4.95 </td></tr><tr ><td> TNF? </td><td> pg/ml </td><td> 4000 � 5.49 </td><td> 5.20 </td></tr></tbody></table> <h2>Kit Components</h2><p>Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.</p><p>Reagents Include:</p><p>Calibrator: Lyophilized, recombinant antigens in a buffered protein base<br>Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives <br>Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide <br>Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer <br>Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives <br>Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase<br>Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant<br>Plate Seals (20): Adhesive strips</p> |
