| Description: | <h2>Product Overview</h2><p>The Multiplex Mouse Cytokine � Inflammation (14-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 14 biomarkers or analytes. Using less than 50 �l of sample per well, up to 80 samples can be assayed for all 14 markers in the panel within 2.25 hours. The Multiplex Mouse Cytokine � Inflammation (14-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 14 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.</p> <h2>Assay Ranges</h2><table class=attributes> <thead><tr><th></th><th></th><th></th><th></th></tr></thead><tbody><tr ><td></td><td> Units </td><td> Calibrator Range </td><td> LLD Limit </td></tr><tr ><td> GMCSF </td><td> pg/ml </td><td> 2000 - 2.74 </td><td> 2.30 </td></tr><tr ><td> IL-1? </td><td> pg/ml </td><td> 5000 - 6.86 </td><td> 4.20 </td></tr><tr ><td> IL-1? </td><td> pg/ml </td><td> 14000 - 19.20 </td><td> 12.41 </td></tr><tr ><td> IL-2 </td><td> pg/ml </td><td> 5000-6.86 </td><td> 3.44 </td></tr><tr ><td> IL-3 </td><td> pg/ml </td><td> 5000 - 6.86 </td><td> 3.18 </td></tr><tr ><td> IL-4 </td><td> pg/ml </td><td> 5000 - 6.86 </td><td> 2.50 </td></tr><tr ><td> IL-6 </td><td> pg/ml </td><td> 5000 - 6.86 </td><td> 2.90 </td></tr><tr ><td> IL-10 </td><td> pg/ml </td><td> 2000 - 2.75 </td><td> 3.00 </td></tr><tr ><td> IL-12p70 </td><td> pg/ml </td><td> 12000 - 16.46 </td><td> 12.20 </td></tr><tr ><td> IL-17 </td><td> pg/ml </td><td> 10000 - 13.72 </td><td> 6.20 </td></tr><tr ><td> MCP-1 </td><td> pg/ml </td><td> 3000 - 4.12 </td><td> 3.40 </td></tr><tr ><td> MIP-1? </td><td> pg/ml </td><td> 5000 - 6.86 </td><td> 0.61 </td></tr><tr ><td> RANTES </td><td> pg/ml </td><td> 3000 - 4.12 </td><td> 2.15 </td></tr><tr ><td> TNF? </td><td> pg/ml </td><td> 3000 - 4.12 </td><td> 3.40 </td></tr></tbody></table> <h2>Kit Components</h2><p>Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.</p><p>Reagents Include:</p><p>Calibrator: Lyophilized, recombinant antigens in a buffered protein base<br>Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives <br>Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide <br>Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer <br>Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives <br>Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase<br>Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant<br>Plate Seals (20): Adhesive strips</p> |
