| Dilutions: | Cell Counting Kit-8 (CCK-8) is designed to detect cell proliferation and cell toxicity based on WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan). The amount of the formazan in cells is directly proportional to the number of living cells. |
| Description: | Economical Cell Counting Kit-8 (CCK-8) is designed for detecting Cell Proliferation and Cytotoxicity |
| Further Information: | Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8.[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells. The product (formazan) produced by WST-8 is water soluble, no organic solvents or isotopes required. And the formazan is stable and safe. The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1. |
| Shipping Conditions: | Ship on cold packs |
| Storage: | CCK-8 is stable for one year at 0-5 C with protection from light. Store it at -20 C for longer storage. Repeated thawing and freezing causes an increase in the background, which interferes with the assay. Please store the kit at 0-5 C for frequent use. |
| Usage: | Research use only |
