| Applications: | Bioactivity, SDS-PAGE |
| Species Reactivity: | Human |
| MW: | 17.2kDa (150aa), confirmed by MALDI-TOF. |
| Purity: | > 95% by SDS - PAGE |
| Description: | Interleukin-4 (IL-4) is a pleiotropic cytokine that has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of naive helper T-cell to Th2 cells. Interleukin-4 is a co-stimulator of DNA-synthesis and is induces the expression of class II MHC molecules on resting B-cells. It also enhances both secretion and cell surface expression of IgE and IgG1. Recombinant Interleukin-4 protein, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer. |
| Further Information: | Sequence: MGSSHHHHHH SSGLVPRGSH MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS |
| Synonym: | IL-4, BSF-1, Binetrakin, Pitrakinara, Lymphocyte stimulatory factor 1, IL4, interleukin 4, BSF1, BCGF1 |
| Ascension Number: | P05112 |
| NCBI EntrezGene: | NP_000580 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
