WB analysis of whole cell extracts (30 ug lysate)of NIH/3T3 (Lane 1)treated for 20 minutes with 200 nM of PMA (Lane 2),Jurkat (Lane 3)treated for 20 minutes with 200 nM of PMA (Lane 4)using GTX25246 NFAT1 (phospho Ser54)antibody.Dilution :1:500-1:2000