Sandwich ELISA analysis of serial diluted human IL-1 alpha recombinant protein(50ul per well)using GTX15754 IL1 alpha antibody [ILA9-H18] (coating antibody)at 3 ug/ml and GTX15753 IL1 alpha antibody [ILA8-H12] (Biotin)(detecting antibody)at 0.15 ug/mL.