Applications: | WB, ICC/IF, IHC-P, FACS |
Species Reactivity: | Human, Mouse, Rat |
Dilutions: | WB: 1-2 ug/ml. ICC/IF: 1-2 ug/ml. IHC-P: 0.25-0.5 ug/ml. FACS: 1-2 ug/million cells. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95 C followed by cooling at RT for 20 minutes.. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications. |
Isotype: | Rabbit IgG |
Immunogen: | Solubilized keratin extract from human stratum corneum |
Purification: | Protein A/G purified |
Conjugation: | Unconjugated |
Further Information: | Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq |
Synonym: | keratin 76 , HUMCYT2A , KRT2B , KRT2P |
NCBI EntrezGene: | 51350 |
Shipping Conditions: | Ship on cold packs |
Storage: | Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4 C. |
Usage: | Research use only |