Applications: | WB, ICC/IF, IHC-P, FACS, ELISA |
Species Reactivity: | Human, Mouse, Rat |
Dilutions: | WB: 1-2 ug/ml. ICC/IF: 1-2 ug/ml. IHC-P: 1-2 ug/ml. FACS: 1-2 ug/million cells. ELISA: 2-4 ug/ml (for coating). Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95 C followed by cooling at RT for 20 minutes.. For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications. |
Isotype: | Rabbit IgG |
Immunogen: | Recombinant human full-length CD86 protein |
Purification: | Protein A/G purified |
Conjugation: | Unconjugated |
Further Information: | This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymph |
Synonym: | CD86 molecule , B7-2 , B7.2 , B70 , CD28LG2 , LAB72 |
NCBI EntrezGene: | 942 |
Shipping Conditions: | Ship on cold packs |
Storage: | Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4 C. |
Usage: | Research use only |