DF6850 staining RAW264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.
DF6850 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22C.
Western blot analysis of extracts from various samples, using TNFAIP3 Antibody. Lane 1: Hela treated with blocking peptide; Lane 2: Hela; Lane 3: HepG2.