Western blot analysis of extracts from A549 cells, using 5-HT-6 antibody.
DF3505 staining A549 by IF/ICC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod