Western blot analysis of extracts from 293 cells, treated with insulin (0.01U/ml, 15mins), using 5-HT-4 antibody.
DF3503 staining 293 cells by IF/ICC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod