DF3500 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.
DF3500 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22C.
Western blot analysis of extracts from HepG2, using 5-HT-2B Antibody.