Western blot analysis of extracts from mouse lung, using 5-HT-1F antibody.
DF3499 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.
Western blot analysis of extracts from Hela,HepG2,rat brain, using 5-HT-1F Antibody.