HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SEC11A (Cat# RC204971, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC11A (Cat# TA811462)(1:2000). Positive lysates [LY402308] (100 ug) and [LC402308] (20 ug) can be purchased separately from OriGene.