HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SF3B14 (Cat# RC201866, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SF3B14 (Cat# TA811148)(1:2000). Positive lysates [LY414228] (100 ug) and [LC414228] (20 ug) can be purchased separately from OriGene.